This data set consists of vertical profiles of water vapor (H2O) and its isotope ratio D/H expressed as δD(H2O) measured in-situ by the ISOWAT-II diode-laser spectrometer aboard an aircraft. The measurements were made between 150 and about 7000 m a.s.l., with a vertical resolution of 3 m, and during 7 days in July and August 2013 over the subtropical North Atlantic Ocean near Tenerife. The measurements were realized in the framework of the MUSICA (MUlti-platform remote Sensing of Isotopologues for investigating the Cycle of Atmospheric water) project.